cell line hek293 bcrc 60019 Search Results


hek293 (bcrc 60019  (BioResource International Inc)
90
BioResource International Inc hek293 (bcrc 60019
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, <t>HEK293</t> and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)
Hek293 (Bcrc 60019, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hek293 (bcrc 60019 - by Bioz Stars, 2026-08
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hek 293  (ATCC)
92
ATCC hek 293
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, <t>HEK293</t> and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)
Hek 293, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
hek 293 - by Bioz Stars, 2026-08
92/100 stars
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a-549 (bcrc 60074  (BioResource International Inc)
90
BioResource International Inc a-549 (bcrc 60074
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, <t>HEK293</t> and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)
A 549 (Bcrc 60074, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
a-549 (bcrc 60074 - by Bioz Stars, 2026-08
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cell line h184b5f5/m10 bcrc 60197  (BioResource International Inc)
90
BioResource International Inc cell line h184b5f5/m10 bcrc 60197
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, <t>HEK293</t> and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)
Cell Line H184b5f5/M10 Bcrc 60197, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+hek293+bcrc+60019/pmc05924487-64-17-23?v=BioResource+International+Inc
Average 90 stars, based on 1 article reviews
cell line h184b5f5/m10 bcrc 60197 - by Bioz Stars, 2026-08
90/100 stars
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cell line ags bcrc 60102  (BioResource International Inc)
90
BioResource International Inc cell line ags bcrc 60102
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, <t>HEK293</t> and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)
Cell Line Ags Bcrc 60102, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+hek293+bcrc+60019/pmc05924487-64-1-23?v=BioResource+International+Inc
Average 90 stars, based on 1 article reviews
cell line ags bcrc 60102 - by Bioz Stars, 2026-08
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ht-29 (human colon cancer cell line, bcrc 60157)  (BioResource International Inc)
90
BioResource International Inc ht-29 (human colon cancer cell line, bcrc 60157)
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, <t>HEK293</t> and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)
Ht 29 (Human Colon Cancer Cell Line, Bcrc 60157), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ht-29 (human colon cancer cell line, bcrc 60157) - by Bioz Stars, 2026-08
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bovine serum  (GE Healthcare)
96
GE Healthcare bovine serum
Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, <t>HEK293</t> and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)
Bovine Serum, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+hek293+bcrc+60019/pmc08195997-188-37-41?v=GE+Healthcare
Average 96 stars, based on 1 article reviews
bovine serum - by Bioz Stars, 2026-08
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Image Search Results


Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, HEK293 and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)

Journal: BMC Cancer

Article Title: Development of a computational promoter with highly efficient expression in tumors

doi: 10.1186/s12885-018-4421-7

Figure Lengend Snippet: Expression level of transcription factors in different cells. a The mRNA levels of NF-κB, CREB and HIF-1α in B16F10, Balb3T3, HT29, HEK293 and HUVECs were determined by RT-PCR. The loading control for B16F10 and Balb3T3 cells was mouse β-actin, and the loading control for HT29, HEK293 and HUVECs was human GAPDH. b Protein expression levels of five cell lines were verified by western blotting. c and d In vitro verification of the D5 promoter activity in tumor and normal cell lines. pARE-hrGFP, pHIF-1α-hrGFP, pNFκB-hrGFP, pCRE-hrGFP and pD5-hrGFP were separately transfected into different cells. Twenty-four hours after transfection, the cell lines were treated with control medium (close column) or activators (open column). After the treatments, the green fluorescence levels were determined by a flow cytometer. The data were analyzed from three independent experiments, and statistically significant differences were determined by t-test (* p < 0.05)

Article Snippet: The human cell lines MCF-7 (BCRC 60436), A-549 (BCRC 60074), AGS (BCRC 60102), HEK293 (BCRC 60019), and H184B5F5/M10 (BCRC 60197) were obtained from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, In Vitro, Activity Assay, Transfection, Fluorescence, Flow Cytometry